Overview

  • Product name
  • Description
    Rabbit polyclonal to CEP128
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CEP128 aa 913-943 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6ZU80

  • Positive control
    • HL60 cell lysate, Human testis tissue, HL60 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 128 kDa.

Target

  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Associates with the mother centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf145 antibody
    • C14orf61 antibody
    • CE128_HUMAN antibody
    • Centrosomal protein 128kDa antibody
    • Centrosomal protein of 128 kDa antibody
    • Cep128 antibody
    • LEDP/132 antibody
    • lens epithelium-derived protein antibody
    see all

Images

  • Anti-CEP128 antibody (ab175959) at 1/1000 dilution + HL60 cell lysate at 35 µg

    Predicted band size : 128 kDa
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue, labeling CEP128 using ab175959 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometry analysis of HL60 cells labeling CEP128  (green, bottom histogram), using ab175959 at a 1/10 dilution, and negative control cells (blue, top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175959 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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