• Product nameAnti-CEP135 antibody
    See all CEP135 primary antibodies
  • Description
    Rabbit polyclonal to CEP135
  • Specificityab75005 detects endogenous levels of total CEP135 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    synthesized peptide derived from C-terminal region of human CEP135.

  • Positive control
    • extracts from COLO cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab75005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/40000.


  • FunctionCentrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
  • Involvement in diseaseMicrocephaly 8, primary, autosomal recessive
  • Sequence similaritiesBelongs to the CEP135/TSGA10 family.
  • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomal protein 135 kDa antibody
    • centrosomal protein 135kDa antibody
    • Centrosomal protein 4 antibody
    • Centrosomal protein of 135 kDa antibody
    • centrosome protein 4 antibody
    • centrosome protein cep135 antibody
    • Cep135 antibody
    • CEP4 antibody
    • CP135_HUMAN antibody
    see all

Anti-CEP135 antibody images

  • All lanes : Anti-CEP135 antibody (ab75005) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 134 kDa
    Observed band size : 134 kDa

References for Anti-CEP135 antibody (ab75005)

This product has been referenced in:
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »
  • Sawant DB  et al. Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function. Mol Biol Cell 26:3741-53 (2015). Read more (PubMed: 26354417) »

See all 5 Publications for this product

Product Wall

Thanks for your reply.
It does seem that the vial of ab75005 you received is not working as expected. I am very sorry for the trouble. If you have received this antibody within the past 6 months or so I would be happy to offer a free replacement o...

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Thank you for your enquiry. I am sorry to hear this vial of ab75005 is giving you trouble.
To ensure that the cell lysates are not degraded, I was wondering if you had successfully blotted these same cell lysates with any other antibodies?

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

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Submitted Jul 29 2010