Customer reviews and FAQs

Overview

  • Product name
    Anti-CEP135 antibody
    See all CEP135 primary antibodies
  • Description
    Rabbit polyclonal to CEP135
  • Specificity
    ab75005 detects endogenous levels of total CEP135 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    synthesized peptide derived from C-terminal region of human CEP135.

  • Positive control
    • extracts from COLO cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/40000.

Target

  • Function
    Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
  • Involvement in disease
    Microcephaly 8, primary, autosomal recessive
  • Sequence similarities
    Belongs to the CEP135/TSGA10 family.
  • Cellular localization
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • Database links
    • Alternative names
      • centrosomal protein 135 kDa antibody
      • centrosomal protein 135kDa antibody
      • Centrosomal protein 4 antibody
      • Centrosomal protein of 135 kDa antibody
      • centrosome protein 4 antibody
      • centrosome protein cep135 antibody
      • Cep135 antibody
      • CEP4 antibody
      • CP135_HUMAN antibody
      see all

    Anti-CEP135 antibody images

    • Western blot - CEP135 antibody (ab75005)
      Western blot - CEP135 antibody (ab75005)
      All lanes : Anti-CEP135 antibody (ab75005) at 1/500 dilution

      Lane 1 : extracts from COLO cells
      Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 134 kDa
      Observed band size : 134 kDa

    References for Anti-CEP135 antibody (ab75005)

    This product has been referenced in:
    • Flanagan AM  et al. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification. Mol Biol Cell 28:736-745 (2017). Read more (PubMed: 28100636) »
    • Sgourdou P  et al. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Sci Rep 7:43708 (2017). Read more (PubMed: 28272472) »

    See all 14 Publications for this product

    Publishing research using ab75005? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab75005 Submit a question for ab75005

    3 Abreviews or Q&A

    10
    Votes: Highest First


    We have tested the lysates with tubulin as the control.

    Thanks for your reply.
    It does seem that the vial of ab75005 you received is not working as expected. I am very sorry for the trouble. If you have received this antibody within the past 6 months or so I would be happy to offer a free replacement o...

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    Inquiry: We have run a numer of western blots with Hela lysate, U2OS lysate and RPE-1 lysate. We are getting a band between 120kDa and 85kDa, a band between 50kDa and 40kDa, a band between 40kDa and 25kDa and a band just under 20kDa. This does not matc...

    Read More

    Thank you for your enquiry. I am sorry to hear this vial of ab75005 is giving you trouble.
    To ensure that the cell lysates are not degraded, I was wondering if you had successfully blotted these same cell lysates with any other antibodies?
    Th...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CEP135 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH3T3)
    Specification
    NIH3T3
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jul 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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