Overview

  • Product name
  • Description
    Rabbit polyclonal to CEP135
  • Specificity
    ab75005 detects endogenous levels of total CEP135 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    synthesized peptide derived from C-terminal region of human CEP135.

  • Positive control
    • extracts from COLO cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/40000.

Target

  • Function
    Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
  • Involvement in disease
    Microcephaly 8, primary, autosomal recessive
  • Sequence similarities
    Belongs to the CEP135/TSGA10 family.
  • Cellular localization
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomal protein 135 kDa antibody
    • centrosomal protein 135kDa antibody
    • Centrosomal protein 4 antibody
    • Centrosomal protein of 135 kDa antibody
    • centrosome protein 4 antibody
    • centrosome protein cep135 antibody
    • Cep135 antibody
    • CEP4 antibody
    • CP135_HUMAN antibody
    see all

Anti-CEP135 antibody images

  • All lanes : Anti-CEP135 antibody (ab75005) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 134 kDa
    Observed band size : 134 kDa

References for Anti-CEP135 antibody (ab75005)

This product has been referenced in:
  • Flanagan AM  et al. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification. Mol Biol Cell 28:736-745 (2017). Read more (PubMed: 28100636) »
  • Sgourdou P  et al. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Sci Rep 7:43708 (2017). Read more (PubMed: 28272472) »

See all 14 Publications for this product

Product Wall

Thanks for your reply.
It does seem that the vial of ab75005 you received is not working as expected. I am very sorry for the trouble. If you have received this antibody within the past 6 months or so I would be happy to offer a free replacement o...

Read More

Thank you for your enquiry. I am sorry to hear this vial of ab75005 is giving you trouble.
To ensure that the cell lysates are not degraded, I was wondering if you had successfully blotted these same cell lysates with any other antibodies?
Th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up