- Datasheet
- Specific References (5)
- Protocols
Overview
- Product nameAnti-CEP135 antibody
See all CEP135 primary antibodies - DescriptionRabbit polyclonal to CEP135
- Specificityab75005 detects endogenous levels of total CEP135 protein.
- Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
- Species reactivityReacts with: Mouse, Human
- Immunogen
synthesized peptide derived from C-terminal region of human CEP135.
- Positive control
- extracts from COLO cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab75005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 134 kDa. | |
| ELISA | 1/40000. |
Target
- FunctionCentrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
- Involvement in diseaseMicrocephaly 8, primary, autosomal recessive
- Sequence similaritiesBelongs to the CEP135/TSGA10 family.
- Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
- Information by UniProt
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Database links
- Entrez Gene: 9662 Human
- Entrez Gene: 381644 Mouse
- Omim: 611423 Human
- SwissProt: Q66GS9 Human
- SwissProt: Q6P5D4 Mouse
- Unigene: 518767 Human
- Unigene: 332452 Mouse
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Alternative names
- centrosomal protein 135 kDa antibody
- centrosomal protein 135kDa antibody
- Centrosomal protein 4 antibody
see all
Anti-CEP135 antibody images
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Western blot - CEP135 antibody (ab75005)All lanes : Anti-CEP135 antibody (ab75005) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 134 kDa
Observed band size : 134 kDa
References for Anti-CEP135 antibody (ab75005)
This product has been referenced in:
- Shiratsuchi G et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »
- Sawant DB et al. Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function. Mol Biol Cell 26:3741-53 (2015). Read more (PubMed: 26354417) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"