Overview

  • Product name
  • Description
    Rabbit polyclonal to CEP170
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 1534 and the C-terminus (residue 1584) of human CEP170 (NP_055627.2)

  • Positive control
    • HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500. Permeabilization with Triton-X 100 is recommended.

Target

  • Relevance
    CEP170, centrosomal protein 170kDa, plays a role in microtubule organization. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes.
  • Cellular localization
    Centrosome, centriole. Cytoplasm, cytoskeleton. Associated with the mature mother centriole. Associated with spindle microtubules during mitosis.
  • Database links
  • Alternative names
    • XRCC5 binding protein antibody
    • 4933426L22Rik antibody
    • A330004A13Rik antibody
    • AI195353 antibody
    • Centrosomal protein 170kDa antibody
    • Cep170 antibody
    • FAM68A antibody
    • KAB antibody
    • KARP 1 binding protein antibody
    • KARP1 binding protein antibody
    • KIAA0470 antibody
    see all

Images

  • Detection of Human CEP170 by Immunocytochemistry.
    Sample: NBF-fixed asynchronous HeLa cells.
    Antibody: ab84545 used at a dilution of 1/100.
    Detection: Red-fluorescent Alexa Fluor 594 goat anti-rabbit IgG.
  • Detection of Human CEP170 by Immunocytochemistry.
    Sample: NBF-fixed asynchronous HeLa cells.
    Antibody: ab84545 used at a dilution of 1/100.
    Detection: Red-fluorescent Alexa Fluor 594 goat anti-rabbit IgG.

References

This product has been referenced in:
  • Hossain M & Stillman B Meier-Gorlin syndrome mutations disrupt an Orc1 CDK inhibitory domain and cause centrosome reduplication. Genes Dev 26:1797-810 (2012). Read more (PubMed: 22855792) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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