Overview

  • Product nameAnti-CEP55 antibody
    See all CEP55 primary antibodies
  • Description
    Rabbit polyclonal to CEP55
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (TLDFENEKLD RQHVQHQLHV ILKELRKARN QITQLESLKQ LHEFAITEPL) of Human CEP55 (NP_060601).

  • Positive control
    • Human fetal lung lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPlays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
  • Tissue specificityWidely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
  • Post-translational
    modifications
    There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > centrosome. Cleavage furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • C10orf3 antibody
    • cancer/testis antigen 111 antibody
    • Centrosomal protein 55kDa antibody
    • Centrosomal protein of 55 kDa antibody
    • CEP 55 antibody
    • Cep55 antibody
    • CEP55_HUMAN antibody
    • CT111 antibody
    • FLJ10540 antibody
    • Up regulated in colon cancer 6 antibody
    • Up-regulated in colon cancer 6 antibody
    • URCC 6 antibody
    • URCC6 antibody
    see all

Anti-CEP55 antibody images

  • Anti-CEP55 antibody (ab84580) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-CEP55 antibody (ab84580)

ab84580 has not yet been referenced specifically in any publications.

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