Overview

  • Product nameAnti-CEP55 antibody
    See all CEP55 primary antibodies
  • Description
    Rabbit polyclonal to CEP55
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the N-terminal region of Human CEP55 conjugated to KLH (NP_001120654.1).

  • Positive control
    • MDA-MB231 cell line lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab91469 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
    • Tissue specificityWidely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
    • Post-translational
      modifications
      There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > centrosome. Cleavage furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • C10orf3 antibody
      • cancer/testis antigen 111 antibody
      • Centrosomal protein 55kDa antibody
      • Centrosomal protein of 55 kDa antibody
      • CEP 55 antibody
      • Cep55 antibody
      • CEP55_HUMAN antibody
      • CT111 antibody
      • FLJ10540 antibody
      • Up regulated in colon cancer 6 antibody
      • Up-regulated in colon cancer 6 antibody
      • URCC 6 antibody
      • URCC6 antibody
      see all

    Anti-CEP55 antibody images

    • Anti-CEP55 antibody (ab91469) at 2 µg/ml + MDA-MB231 cell line lysate at 35 µg

      Predicted band size : 54 kDa

    References for Anti-CEP55 antibody (ab91469)

    ab91469 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab91469.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"