Overview

  • Product name
    Anti-CEP55 antibody [EPR11944(B)]
    See all CEP55 primary antibodies
  • Description
    Rabbit monoclonal [EPR11944(B)] to CEP55
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CEP55. The exact sequence is proprietary.
    Database link: Q53EZ4

  • Positive control
    • SW480, HeLa, and HepG2 cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11944(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/5000 - 1/20000. Predicted molecular weight: 55 kDa.

For unpurified use at 1/10000 - 1/50000.

ICC/IF 1/400.

For unpurified use at 1/250 - 1/500.

Flow Cyt 1/170.

For unpurified use at 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
  • Tissue specificity
    Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
  • Post-translational
    modifications
    There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > centrosome. Cleavage furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • C10orf3 antibody
    • cancer/testis antigen 111 antibody
    • Centrosomal protein 55kDa antibody
    • Centrosomal protein of 55 kDa antibody
    • CEP 55 antibody
    • Cep55 antibody
    • CEP55_HUMAN antibody
    • CT111 antibody
    • FLJ10540 antibody
    • Up regulated in colon cancer 6 antibody
    • Up-regulated in colon cancer 6 antibody
    • URCC 6 antibody
    • URCC6 antibody
    see all

Images

  • Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/5000 dilution + SW480 Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

  • Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

  • All lanes : Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa

References

This product has been referenced in:
  • Gao K  et al. iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation. Cell Death Dis 9:528 (2018). Read more (PubMed: 29743530) »
  • Peng T  et al. Centrosomal protein 55 activates NF-?B signalling and promotes pancreatic cancer cells aggressiveness. Sci Rep 7:5925 (2017). IHC . Read more (PubMed: 28724890) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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