1/100 - 1/500. Heat mediated antigen retrieval using 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.
FunctionCentrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2.
Sequence similaritiesBelongs to the translokin family.
DomainThe C-terminal region mediates the interaction with microtubules and is able to nucleate and bundles microtubules in vitro. The centrosome localization domain (CLD) region mediates the localization to centrosomes and homooligomerization.