The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ/PLK1S1, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs).
Belongs to the CEP72 family. Contains 3 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.
Cytoplasm > cytoskeleton > centrosome. Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule-dependent manner.
All lanes : Anti-CEP72 antibody (ab75254) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa Observed band size: 72 kDa
Exposure time: 3 minutes
Immunoprecipitation - CEP72 antibody (ab75254)
Detection of CEP72 by Western Blot of Immunprecipitate.
ab75254, at 1µg/ml, staining CEP72 in HeLa whole cell lysate immunoprecipitated using ab75254 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.