Overview

  • Product name
  • Description
    Rabbit polyclonal to CEP72
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 150 and 200 of human CEP72 protein (NP_060610.2)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab75254 was affinity purified using an epitope specific to CEP72 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ/PLK1S1, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs).
    • Sequence similarities
      Belongs to the CEP72 family.
      Contains 3 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
    • Cellular localization
      Cytoplasm > cytoskeleton > centrosome. Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • Centrosomal protein 72kDa antibody
      • Centrosomal protein of 72 kDa antibody
      • Cep72 antibody
      • CEP72_HUMAN antibody
      • FLJ10565 antibody
      • MGC5307 antibody
      • RGD1566336 antibody
      see all

    Images

    • All lanes : Anti-CEP72 antibody (ab75254) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 72 kDa
      Observed band size : 72 kDa


      Exposure time : 3 minutes
    • Detection of CEP72 by Western Blot of Immunprecipitate.
      ab75254, at 1µg/ml, staining CEP72 in HeLa whole cell lysate immunoprecipitated using ab75254 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab75254 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab75254.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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