Overview

  • Product name
  • Description
    Rabbit polyclonal to CEP78
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 639 and 689 of human CEP78 (CAI40237.1)

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      CEP78 belongs to the CEP78 family and contains 3 LRR (leucine rich) repeats.
    • Cellular localization
      Cytoplasmic; centrosome
    • Database links
    • Alternative names
      • 5730599I05Rik antibody
      • C9orf81 antibody
      • Centrosomal protein 78 antibody
      • Centrosomal protein 78kDa antibody
      • Chromosome 9 open reading frame 81 antibody
      • D030027P05 antibody
      • FLJ12643 antibody
      • FLJ52093 antibody
      • IP63 antibody
      • MGC135040 antibody
      • RP11-336N8.5 antibody
      see all

    Images

    • All lanes : Anti-CEP78 antibody (ab86188) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 76 kDa
      Observed band size : 76 kDa


      Exposure time : 3 minutes
    • Detection of human CEP78 in Immunoprecipitates of HeLa whole cell lysate (1 mg klysate for IP, 20% of IP loaded) using ab86188 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab86188 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86188.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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