Anti-CEP89 antibody - C-terminal (ab170218)

Overview

  • Product name
    Anti-CEP89 antibody - C-terminal
    See all CEP89 primary antibodies
  • Description
    Rabbit polyclonal to CEP89 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CEP89 aa 663-694 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96ST8

  • Positive control
    • Mouse Neuro-2a cell line lysate; Human colon carcinoma tissue; Mouse Neuro-2a cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 90 kDa.

Target

  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Localizes to structures around centrosomes similar to centrosome satellites.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCDC123 antibody
    • Centrosomal protein 89 kDa antibody
    • Centrosomal protein of 89 kDa antibody
    • cep89 antibody
    • CEP89_HUMAN antibody
    • Coiled-coil domain-containing protein 123 antibody
    • Coiled-coil domain-containing protein 123, mitochondrial antibody
    • FLJ14640 antibody
    see all

Images

  • Flow cytometric analysis of Neuro-2a cells cells labeling CEP89 with ab170218 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Iimmunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling CEP89 with ab170218 at 1/10 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-CEP89 antibody - C-terminal (ab170218) at 1/100 dilution + Mouse Neuro-2a cell line lysates at 35 µg

    Predicted band size: 90 kDa

References

ab170218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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