Overview

  • Product name
    Anti-CEPT1 antibody
  • Description
    Rabbit polyclonal to CEPT1
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human CEPT1 aa 29-67 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab107415 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
IP Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.

Target

  • Function
    Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.
  • Tissue specificity
    Ubiquitously expressed.
  • Pathway
    Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.
    Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.
  • Sequence similarities
    Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
  • Cellular localization
    Endoplasmic reticulum membrane. Nucleus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cept1 antibody
    • CEPT1_HUMAN antibody
    • Choline/ethanolamine phosphotransferase 1 antibody
    • Choline/ethanolaminephosphotransferase 1 antibody
    • DKFZp313G0615 antibody
    • hCEPT1 antibody
    • MGC45223 antibody
    see all

Images

  • CEPT1 antibody (N-term) (ab107415) immunohistochemistry analysis in formalin fixed and paraffin embedded Human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-CEPT1 antibody (ab107415) at 1/100 dilution + Cell lysate HL60 at 35 µg

    Predicted band size : 47 kDa

References

ab107415 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Purified protein (Liver)
Loading amount
1000 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 12 2012

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Liver)
Total protein in input
1000 µg
Specification
Liver
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted May 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up