For the best experience on the Abcam website please upgrade to a modern browser such as
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
Found in all tissues examined.
Lipid metabolism; sphingolipid metabolism.
Belongs to the glycosyltransferase 2 family.
Golgi apparatus membrane.
Information by UniProt
Ceramide glucosyltransferase antibody
Anti-Ceramide glucosyltransferase antibody images
Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)
All lanes :
Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml
Lane 1 :
Human brain tissue lysate - total protein (
Lane 2 :
Brain (Mouse) Tissue Lysate
Lane 3 :
Brain (Rat) Tissue Lysate
Lane 4 :
Spleen (Mouse) Tissue Lysate
Lane 5 :
Spleen (Rat) Tissue Lysate
Lane 6 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 :
A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
48 kDa (
why is the actual band size different from the predicted?
Additional bands at :
12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"