Anti-Ceramide glucosyltransferase antibody (ab124296)

Overview

  • Product nameAnti-Ceramide glucosyltransferase antibody
    See all Ceramide glucosyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Ceramide glucosyltransferase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ceramide glucosyltransferase.

  • Positive control
    • This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab124296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionCatalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
  • Tissue specificityFound in all tissues examined.
  • PathwayLipid metabolism; sphingolipid metabolism.
  • Sequence similaritiesBelongs to the glycosyltransferase 2 family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEGT_HUMAN antibody
    • Ceramide glucosyltransferase antibody
    • GCS antibody
    • GLCT 1 antibody
    • GLCT-1 antibody
    • GLCT1 antibody
    • Glucosylceramide synthase antibody
    • UDP glucose ceramide glucosyltransferase antibody
    • UDP glucose N acylsphingosine D glucosyltransferase antibody
    • UDP-glucose ceramide glucosyltransferase antibody
    • UDP-glucose:N-acylsphingosine D-glucosyltransferase antibody
    • Ugcg antibody
    see all

Anti-Ceramide glucosyltransferase antibody images

  • All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Spleen (Rat) Tissue Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-Ceramide glucosyltransferase antibody (ab124296)

ab124296 has not yet been referenced specifically in any publications.

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