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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
Function Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
Tissue specificity Found in all tissues examined.
Pathway Lipid metabolism; sphingolipid metabolism.
Sequence similarities Belongs to the glycosyltransferase 2 family.
Cellular localization Golgi apparatus membrane.
Information by UniProt
Ceramide glucosyltransferase antibody
Anti-Ceramide glucosyltransferase antibody images
Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)
All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml Lane 1 : Human brain tissue lysate - total protein ( ab29466) Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lane 4 : Spleen (Mouse) Tissue Lysate Lane 5 : Spleen (Rat) Tissue Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 45 kDa Observed band size : 48 kDa ( why is the actual band size different from the predicted?) Additional bands at : 12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
References for Anti-Ceramide glucosyltransferase antibody (ab124296)
has not yet been referenced specifically in any publications.
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