Anti-Ceramide synthase 1/LAG1 antibody (ab85696)
Key features and details
- Goat polyclonal to Ceramide synthase 1/LAG1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ceramide synthase 1/LAG1 antibody
See all Ceramide synthase 1/LAG1 primary antibodies -
Description
Goat polyclonal to Ceramide synthase 1/LAG1 -
Host species
Goat -
Specificity
ab85696 is expected to recognize both reported isoforms (NP_067090.1 and NP_937850.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human Ceramide synthase 1/LAG1 aa 318-329 (internal sequence). (NP_937850.1)
Sequence:C-KDLREYDTAEAQ
Database link: NP_067090.1 -
Positive control
- Human brain (cerebellum, cerebral cortex and frontal cortex) lysates.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85696 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa).
Calculated MW of 38.0 kDa according to NP_937850.1. An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide. 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa). Calculated MW of 38.0 kDa according to NP_937850.1. An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide. 1 hour primary incubation is recommended for this product. |
Target
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Function
May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner. -
Sequence similarities
Contains 1 TLC (TRAM/LAG1/CLN8) domain. -
Cellular localization
Endoplasmic reticulum membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Isoform 1 may recycle from the Golgi to the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 615296 Cow
- Entrez Gene: 10715 Human
- Entrez Gene: 93898 Mouse
- Entrez Gene: 290658 Rat
- Omim: 606919 Human
- SwissProt: P27544 Human
- SwissProt: P27545 Mouse
- Unigene: 412355 Human
see all -
Alternative names
- Ceramide synthase 1 antibody
- CerS1 antibody
- CERS1_HUMAN antibody
see all
Images
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Anti-Ceramide synthase 1/LAG1 antibody (ab85696) at 1 µg/ml + Human Frontal cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. An additional band of unknown identity was consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide.
Datasheets and documents
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Datasheet download
References (0)
ab85696 has not yet been referenced specifically in any publications.