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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab85696 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
Function May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner.
Sequence similarities Contains 1 TLC (TRAM/LAG1/CLN8) domain.
Cellular localization Endoplasmic reticulum membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Isoform 1 may recycle from the Golgi to the endoplasmic reticulum.
Information by UniProt
Ceramide synthase 1 antibody
Anti-Ceramide synthase 1 antibody images
Western blot - LASS1 antibody (ab85696)
Anti-Ceramide synthase 1 antibody (ab85696) at 1 µg/ml + Human Frontal cortex lysate (in RIPA buffer) at 35 µg Predicted band size : 40 kDa Observed band size : 37 kDa ( why is the actual band size different from the predicted?) Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Ceramide synthase 1 antibody (ab85696)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"