• Product nameAnti-CERK antibody
    See all CERK primary antibodies
  • Description
    Rabbit polyclonal to CERK
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human CERK aa 487-516 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Mouse heart tissue lysate. A2058 cell lysate. IF/ICC: SKNSH cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, eluted with both high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab38011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.


  • FunctionCatalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
  • Tissue specificityHigh level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
  • Sequence similaritiesContains 1 DAGKc domain.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylsphingosine kinase antibody
    • Ceramide kinase antibody
    • Cerk antibody
    • CERK1_HUMAN antibody
    • dA59H18.2 antibody
    • dA59H18.3 antibody
    • hCERK antibody
    • KIAA1646 antibody
    • Lipid kinase 4 antibody
    • Lipid kinase LK4 antibody
    • LK4 antibody
    see all

Anti-CERK antibody images

  • ICC/IF image of ab38011 stained SKNSH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38011, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CERK antibody (ab38011) at 1/100 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : A2058 cell lysate

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Mouse heart tissue lysate (Lane 1), A2058 cell lysate (Lane 2).

References for Anti-CERK antibody (ab38011)

This product has been referenced in:
  • Gubern A  et al. JNK and Ceramide Kinase Govern the Biogenesis of Lipid Droplets through Activation of Group IVA Phospholipase A2. J Biol Chem 284:32359-69 (2009). WB ; Chinese hamster . Read more (PubMed: 19778898) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (CHO-K1 cells)
Loading amount 20 µg
Specification CHO-K1 cells
Treatment CHO-K1 cells treated with three siRNA duplexes designed to knock down CERK expression
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

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Submitted Jan 16 2009