Overview

  • Product nameAnti-CERKL antibody
    See all CERKL primary antibodies
  • Description
    Rabbit polyclonal to CERKL
  • SpecificityThis antibody detects endogenous levels of total CERKL protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from the internal region of human CERKL.

  • Positive control
    • Extracts from A549 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects bands of approximately 63, 40 and 48 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
    • Tissue specificityIsoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver.
    • Involvement in diseaseDefects in CERKL are the cause of retinitis pigmentosa type 26 (RP26) [MIM:608380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive.
    • Sequence similaritiesContains 1 DAGKc domain.
    • Developmental stageExpressed in fetal lung, kidney and brain.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localizationCytoplasm. Nucleus > nucleolus. Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli and Cytoplasm. Nucleus > nucleolus. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ceramide kinase like protein antibody
      • Ceramide kinase-like protein antibody
      • CERKL antibody
      • CERKL_HUMAN antibody
      • Retinitis pigmentosa 26 (autosomal recessive) antibody
      • RP26 antibody
      see all

    Anti-CERKL antibody images

    • All lanes : Anti-CERKL antibody (ab66007) at 1/500 dilution

      Lane 1 : Extracts from A549 cells
      Lane 2 : Extracts from A549 cells, plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 63 kDa
      Observed band size : 63 kDa
      Additional bands at : 40 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-CERKL antibody (ab66007)

    This product has been referenced in:
    • Jelcick AS  et al. Genetic variations strongly influence phenotypic outcome in the mouse retina. PLoS One 6:e21858 (2011). WB ; Mouse . Read more (PubMed: 21779340) »

    See 1 Publication for this product

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    Merci de votre intérêt pour ab66007.

    Cet anticorps n’a pas encore été testé chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires.<...

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