Anti-Ceruloplasmin antibody (ab110449)

Overview

  • Product nameAnti-Ceruloplasmin antibody
    See all Ceruloplasmin primary antibodies
  • Description
    Rabbit polyclonal to Ceruloplasmin
  • Tested applicationsSuitable for: IHC-FoFr, WB, IHC-FrFl, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human Ceruloplasmin aa 350-450 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab111803)

  • Positive control
    • This antibody gave a positive signal in Human plasma total protein lysate as well as Human liver tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 122 kDa).
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCeruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similaritiesBelongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

Anti-Ceruloplasmin antibody images

  • Adult rat was perfused with 4% PFA in 0.1M PBS. Fluorescent staining was performed in duodenum sections with anti-Ceruloplasmin (ab110449) primary and Alexa 488 conjugated secondary antibody. This antibody stained gut enterocytes. Nucleus stained with Hoechst at high power magnification.

    See Abreview

  • All lanes : Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 148 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,75 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml + Natural Human Ceruloplasmin protein (ab77930) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Anti-Ceruloplasmin antibody (ab110449)

ab110449 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions Reduced Denaturing (8)
Loading amount 30 µg
Specification endometrial tissue lysate
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 26 2015

Thank you for your comment.

Before offering a testing discount for new species, our Scientific Team need to check the sequence homology to see if the cross-reactivity is expected or not.

If the homology is higher than 95%, then ther...

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Thank you for the reply.

I have checked the sequence homology between human and pig (Sus scrofa) and it is only 87%. This means that the probability of this antibody to recognize pig (porcine) Ceruloplasmin is low. Please advise your customer...

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Gracias po contactarnos.

En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Duodenum)
Specification Duodenum
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astro-glia)
Specification Astro-glia
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"