Overview

  • Product nameAnti-Ceruloplasmin antibody
    See all Ceruloplasmin primary antibodies
  • Description
    Rabbit polyclonal to Ceruloplasmin
  • Tested applicationsSuitable for: IHC-P, IHC-FoFr, IP, RIA, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human ceruloplasmin purified from human plasma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCeruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similaritiesBelongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

Anti-Ceruloplasmin antibody images

  • Ab48614 staining human tonsil. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-Ceruloplasmin antibody (ab48614) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 122 + 148 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa,76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    Ceruloplasmin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted (148 kDa).
  • IHC-FoFr image of ceruloplasmin staining on mouse duodenal sections using ab48614. These sections were incubated in triton X (0.3% in 0.1% PBS) to permeabilise the cells and in 10% serum for 1h to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab48614(1:200) overnight at +4°C. Specific protien binding was visualised using an Alexa 488 conjugated donkey secondary antibody (green staining). Alexa 568 conjugated secondary was used to visualise ab55027 binding to ferritin (red staining).

    See Abreview

References for Anti-Ceruloplasmin antibody (ab48614)

This product has been referenced in:
  • Dai L  et al. SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin. Nat Commun 7:11996 (2016). WB, IHC-Fr, ICC/IF . Read more (PubMed: 27353863) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing (10% PAGE)
Sample Fish Serum (plasma)
Specification plasma
Blocking step (agent) for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 26 2015

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, ...

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Thank you for your enquiry and your interest in our products.

This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have cont...

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Gracias po contactarnos.

En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Cell (Duodenum)
Specification Duodenum
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"