Overview

  • Product name
    CETP Activity Assay Kit II (Fluorometric)
  • Detection method
    Fluorescent
  • Sample type
    Serum, Plasma, Purified protein
  • Assay type
    Enzyme activity
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mammal
  • Product overview

    CETP Activity Assay Kit II (Fluorometric) (ab196995) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Cholesteryl Ester Transfer Protein (CETP). CETP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 480/511 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum and torcetrapib (CETP inhibitor) as positive and negative control, respectively, for assay validation.

    This assay can measure PLTP activity in plasma and serum but can also be used for testing activity of recombinant CETP protein.

  • Notes

    Cholesteryl Ester Transfer Protein (CETP) is a member of the lipid transfer/lipopolysaccharide binding protein gene family. CETP is plasma protein that transfers a cholesteryl ester from HDL to LDL or VLDL in exchange for a triglyceride. HDL plays an important role in lipid metabolism and cardiovascular health. HDL transports cholesterol to the liver for excretion or to steroidogenic tissues for steroid synthesis. HDL also plays an important role in the reverse cholesterol transport pathway, removing cholesterol from lipid-filled macrophages, protecting against atherosclerosis. Because of this function, CETP is viewed as a target to increase HDL, with CETP inhibition an active area of research and several CETP inhibitors at various stages of drug development.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab196995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Typical Donor molecule Standard Curve obtained following assay protocol.

  • Measurement of CETP activity on 1 µL rabbit serum (dark blue diamonds), 1 µL human plasma (orange squares) or  800 ng recombinant human CETP (purple circles). Background control with no serum is shown (green triangles).

  • Inhibition curve of CETP activity from rabbit serum by Torcetrapib (CETP inhibitor). The assay was run for 1 hour and the IC50 was determined to be 3.56 nM.

  • Inhibition of CETP activity from human plasma using 80 nM Torcetrapib; assay was run for 2 hours following protocol instructions.

Protocols

References

This product has been referenced in:
  • Aghebati T  et al. A novel atheroprotective role of MF59-like adjuvant when co-administered with CETP vaccine in rabbit model of atherosclerosis. Iran J Basic Med Sci 19:1345-1352 (2016). Read more (PubMed: 28096968) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196995.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up