Overview

  • Product nameAnti-CETP antibody [ATM192]
    See all CETP primary antibodies
  • Description
    Mouse monoclonal [ATM192] to CETP
  • SpecificityDetects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.
  • Tested applicationsSuitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide corresponding to Human CETP aa 149-159.
    Sequence:

    CDSGRVRTDAPD

  • Positive control
    • WB,IP: human serum WB: human liver lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. Use protein G beads.
WB 1/400. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/20.

Target

  • FunctionInvolved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
  • Sequence similaritiesBelongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPIFF antibody
    • CETP antibody
    • CETP_HUMAN antibody
    • Cholesteryl ester transfer protein antibody
    • Cholesteryl ester transfer protein plasma antibody
    • HDLCQ10 antibody
    • Lipid transfer protein I antibody
    see all

Anti-CETP antibody [ATM192] images

  • Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Anti-CETP antibody [ATM192] (ab2726) at 1/500 dilution + Whole cell lysate from rabbit fibroblasts at 40 µg

    Secondary
    HRP-conjugated rabbit anti-mouse at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-CETP antibody [ATM192] (ab2726)

This product has been referenced in:
  • Niculescu LS  et al. Apolipoprotein A-I stimulates cholesteryl ester transfer protein and apolipoprotein E secretion from lipid-loaded macrophages; the role of NF-?B and PKA signaling pathways. Biochem Biophys Res Commun 415:497-502 (2011). WB ; Human . Read more (PubMed: 22056562) »
  • Thomas AP  et al. Mouse monoclonal antipeptide antibodies specific for cholesteryl ester transfer protein (CETP). Hybridoma 15:359-64 (1996). Read more (PubMed: 8913785) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human THP-1 macrophages)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 70 µg
Treatment 100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I
Specification Human THP-1 macrophages
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Anca Sima

Verified customer

Submitted Jun 04 2015

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (4-20% tris-glycine)
Sample Cynomolgus Monkey Serum (serum, PBS diluted)
Specification serum, PBS diluted
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Cell lysate - whole cell (Fibroblasts)
Loading amount 40 µg
Specification Fibroblasts
Treatment virus
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2008

I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the...

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I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the...

Read More

The antibody is IgG1. We have not further characterized the antibody in terms of Kappa and Lamda. Here is a refernce for ELISA: A.P. Thomas Mouse Monoclonal Antipeptide Antibodies Specific for Cholesteryl Ester Transfer Protein (CETP). Hybridoma...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"