Overview

  • Product nameAnti-Cezanne antibody
    See all Cezanne primary antibodies
  • Description
    Rabbit polyclonal to Cezanne
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: GSSGTETLEK KKKNSLKSWK GGKEEAAGDG PVSEKPPAES VGNGGSKYSQ, corresponding to internal sequence amino acids 541-590 of Human ZA20D1

  • Positive control
    • Daudi cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHas deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.
  • Tissue specificityWidely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells.
  • Sequence similaritiesBelongs to the peptidase C64 family.
    Contains 1 A20-type zinc finger.
    Contains 1 OTU domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular zinc finger anti NF kappa B protein antibody
    • Cellular zinc finger anti-NF-kappa-B protein antibody
    • CEZANNE antibody
    • HGNC:16683 antibody
    • OTU domain containing protein 7B antibody
    • OTU domain-containing protein 7B antibody
    • OTU7B_HUMAN antibody
    • OTUD7B antibody
    • ZA20D1 antibody
    • Zinc finger A20 domain containing protein 1 antibody
    • Zinc finger A20 domain-containing protein 1 antibody
    • Zinc finger protein Cezanne antibody
    see all

Anti-Cezanne antibody images

  • Anti-Cezanne antibody (ab49264) at 1.25 µg/ml + Daudi cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 94 kDa
    Observed band size : 94 kDa
    Additional bands at : 40 kDa (possible cross reactivity),80 kDa (possible cross reactivity).

References for Anti-Cezanne antibody (ab49264)

ab49264 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"