Overview

  • Product name
    Anti-CFHR5 antibody
  • Description
    Rabbit polyclonal to CFHR5
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CFHR5 aa 210-239 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (NP_110414.1)
    Database link: Q9BXR6

  • Positive control
    • Human CEM and K562 cell lysates, Human hepatocarcinoma tissue and CEM cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175254 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 64 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in complement regulation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Note=Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFHL5 antibody
    • CFHR5 antibody
    • CFHR5D antibody
    • Complement factor H-related 5 antibody
    • Complement factor H-related protein 5 antibody
    • factor H-related gene 5 antibody
    • factor H-related protein 5 antibody
    • FHR-5 antibody
    • FHR5 antibody
    • FHR5_HUMAN antibody
    • FLJ10549 antibody
    • MGC133240 antibody
    • OTTHUMP00000034672 antibody
    see all

Images

  • All lanes : Anti-CFHR5 antibody (ab175254) at 1/100 dilution

    Lane 1 : CEM cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 64 kDa
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human hepatocarcinoma tissue, labeling CFHR5 using ab175254 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometry analysis of CEM cells labeling CFHR5 (green, right histogram), using ab175254 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Zhai YL  et al. Rare Variants in the Complement Factor H-Related Protein 5 Gene Contribute to Genetic Susceptibility to IgA Nephropathy. J Am Soc Nephrol 27:2894-905 (2016). Read more (PubMed: 26825529) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175254.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up