1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Involved in complement regulation.
Expressed by the liver and secreted in plasma.
Involvement in disease
Note=Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human hepatocarcinoma tissue, labeling CFHR5 using ab175254 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometry - Anti-CFHR5 antibody (ab175254)
Flow cytometry analysis of CEM cells labeling CFHR5 (green, right histogram), using ab175254 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Zhai YL et al. Rare Variants in the Complement Factor H-Related Protein 5 Gene Contribute to Genetic Susceptibility to IgA Nephropathy. J Am Soc Nephrol27:2894-905 (2016).
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