Overview

  • Product nameCFSE - Cell Labeling Kit
    See all Cell proliferation kits
  • Detection methodFluorescent
  • Assay typeCell-based
  • Product overview

    CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.

    CFSE (5(6)-Carboxyfluorescein N-hydroxysuccinimidyl ester) is a cell permeant, non-fluorescent pro-dye. Intracellular esterases in live cells cleave the acetate groups which results in the green fluorescent molecule carboxyfluorescein that is now membrane impermeant. The succinimidyl ester group reacts indiscriminately with intracellular free amines to generate covalent dye-protein conjugates. The result is live cells with an intracellular fluorescent label. At appropriate concentrations CFSE is non-toxic to cells and the fluorescence is retained after formaldehyde and alcohol fixation. CFSE labeled cells can be detected with any instrument or filter set compatible with fluroescein detection: Excitation(max)=492nm, Emission(max)=517nm.

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformReagents

Properties

  • RelevanceCell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.

Applications

Our Abpromise guarantee covers the use of ab113853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

CFSE - Cell Labeling Kit images

  • Jurkat cells labeled with ab113853 (CFSE). Jurkat cells were labeled with 1mM CFSE in media for 15 minutes, washed once with PBS and imaged on a flurorescence microscope. The cells in this image are live but fixed cells give similar results.
  • Flow cytometry analysis of ab113853 (CFSE) dilution with cell division. Jurkat cells were labeled with 1µM CFSE on day0 and then a portion of the culture was subjected to flow cytometry analysis on days 1-7. Shown, an overlay histogram of the daily CFSE fluorescence intensity.
  • Duplexing treated and untreated cells in a single sample. One plate of HeLa cells was labeled with ab113853 (CFSE) and a second plate was treated with the hypoxia mimetic deferoxamine (DFO) but not labeled with CFSE. After harvesting, the cells were combined in a single tube, stained with a HIF1A antibody and subjected to flow cytometry. Expected results are shown in the left panel cartoon. The no primary antibody control sample (middle panel) shows the resolution of CFSE labeled and unlabeled cells. The HIF1A antibody stained cells (right panel) shows that only cells exposed to DFO have an increase in HIF1A protein levels.

Protocols

References for CFSE - Cell Labeling Kit (ab113853)

ab113853 has not yet been referenced specifically in any publications.

Product Wall



I contacted the laboratories to discuss this further. They also suggest that the CFSE - Cell Labeling Kit would be easier to use than BrdU.

1. CFSE versus BrdU - these are very different assays.
BrdU is a more direct measure of p...

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CFSE can be fixed with formaldehyde and retain fluorescence (for ICC and flow cytometry).

Unfortunately, as we have not tried it in house we are not able to state whether CFSE fluorescence would survive the paraffin embedding proces...

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Thank you for contacting us.

No stimulation was performed in this assay, simply culturing the cells in standard growth media.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"