Anti-CFTR antibody (ab59394)
Key features and details
- Rabbit polyclonal to CFTR
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CFTR antibody
See all CFTR primary antibodies -
Description
Rabbit polyclonal to CFTR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized non-phosphopeptide derived from human CFTR around the phosphorylation site of serine 737.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (3) |
1/50 - 1/100.
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Notes |
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IHC-P
1/50 - 1/100. |
Target
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Function
Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. -
Tissue specificity
Found on the surface of the epithelial cells that line the lungs and other organs. -
Involvement in disease
Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Domain
The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex. -
Post-translational
modificationsPhosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling. -
Cellular localization
Early endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1080 Human
- Omim: 602421 Human
- SwissProt: P13569 Human
- Unigene: 489786 Human
- Unigene: 621460 Human
- Unigene: 661104 Human
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Alternative names
- ABC 35 antibody
- ABC35 antibody
- ABCC 7 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab59394 has been referenced in 9 publications.
- Duclaux-Loras R et al. UNC45A deficiency causes microvillus inclusion disease-like phenotype by impairing myosin VB-dependent apical trafficking. J Clin Invest 132:N/A (2022). PubMed: 35575086
- Abtahi S et al. A Simple Method for Creating a High-Content Microscope for Imaging Multiplexed Tissue Microarrays. Curr Protoc 1:e68 (2021). PubMed: 33822482
- Scaffa A et al. Single-cell transcriptomics reveals lasting changes in the lung cellular landscape into adulthood after neonatal hyperoxic exposure. Redox Biol 48:102091 (2021). PubMed: 34417156
- Oliver KE et al. Slowing ribosome velocity restores folding and function of mutant CFTR. J Clin Invest 129:5236-5253 (2019). PubMed: 31657788
- Yoshie S et al. Functional characterization of various channel-expressing central airway epithelial cells from mouse induced pluripotent stem cells. J Cell Physiol N/A:N/A (2019). PubMed: 30714154
- Matsui S et al. Characterization of Peribiliary Gland-Constituting Cells Based on Differential Expression of Trophoblast Cell Surface Protein 2 in Biliary Tract. Am J Pathol 188:2059-2073 (2018). PubMed: 30126547
- Liu Y et al. Cystic fibrosis transmembrane conductance regulator mediates tenogenic differentiation of tendon-derived stem cells and tendon repair: accelerating tendon injury healing by intervening in its downstream signaling. FASEB J 31:3800-3815 (2017). PubMed: 28495756
- Brennan SC et al. The extracellular calcium-sensing receptor regulates human fetal lung development via CFTR. Sci Rep 6:21975 (2016). PubMed: 26911344
- Borth H et al. The IP3 R Binding Protein Released With Inositol 1,4,5-Trisphosphate Is Expressed in Rodent Reproductive Tissue and Spermatozoa. J Cell Physiol 231:1114-29 (2016). PubMed: 26439876