Overview

  • Product name
  • Description
    Rabbit polyclonal to CGBP
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: VWYKLDELFE QERNVRTAMT NRAGLLALML HQTIQHDPLT TDLRSSADR, corresponding to amino acids 608-656 of Human CGBP

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab56035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 76 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration. PubMed: 21828268

Target

  • Function
    Transcriptional activator that exhibits a unique DNA binding specificity for [AC]CpG[AC] unmethylated CpG motifs.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 CXXC-type zinc finger.
    Contains 1 PHD-type zinc finger.
  • Domain
    The acidic domain carries the potential to activate transcription.
  • Post-translational
    modifications
    May be regulated by proteolysis.
  • Cellular localization
    Nucleus speckle. Associated with euchromatin. During mitosis, excluded from condensed chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410002I16Rik antibody
    • 5830420C16Rik antibody
    • AI426635 antibody
    • CFP 1 antibody
    • CFP1 antibody
    • CpG binding protein antibody
    • CpG-binding protein antibody
    • CXXC 1 antibody
    • CXXC finger 1 antibody
    • CXXC finger 1 PHD domain antibody
    • CXXC finger protein 1 antibody
    • CXXC type zinc finger protein 1 antibody
    • CXXC-type zinc finger protein 1 antibody
    • CXXC1 antibody
    • CXXC1_HUMAN antibody
    • DNA binding protein with PHD finger and CXXC domain antibody
    • hCGBP antibody
    • HsT2645 antibody
    • PCCX 1 antibody
    • PCCX1 antibody
    • PHD finger and CXXC domain containing protein 1 antibody
    • PHD finger and CXXC domain-containing protein 1 antibody
    • PHF 18 antibody
    • PHF18 antibody
    • Protein containing CXXC domain 1 antibody
    • SPP 1 antibody
    • SPP1 antibody
    • ZCGPC 1 antibody
    • ZCGPC1 antibody
    • Zinc finger, CpG binding type containing 1 antibody
    see all

Images

  • Anti-CGBP antibody (ab56035) at 0.625 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 76 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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