Recombinant
RabMAb

Anti-cGMP antibody [EPR10355] (ab169753)

Overview

  • Product name
    Anti-cGMP antibody [EPR10355]
    See all cGMP primary antibodies
  • Description
    Rabbit monoclonal [EPR10355] to cGMP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P,IP or WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    cGMP conjugated to KLH.

  • General notes

    The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 0.4 µg/ml.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P,IP or WB.
  • Target

    • Relevance
      Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
    • Alternative names
      • Cyclic GMP antibody
      • Cyclic guanosine monophosphate antibody
      • Guanosine 3 5 Cyclic Monophosphate antibody

    Images

    • 2 µg/ml of Goat anti-rabbit IgG (50 µl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 µg/ml of ab169753 (50 µl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 µl/well) were added along with 25 µl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.
    • 2µg/ml of goat-anti-rabbit IgG (50µl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2µg/ml of ab169753 (50 µl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 µl / well each) were added, respectively. Finally 25 µl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color.

    References

    ab169753 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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