cGMP Assay Kit - Direct Immunoassay (ab65356)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.008 µM
Overview
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Product name
cGMP Assay Kit - Direct Immunoassay -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
> 0.008 µM -
Range
0.008 µM - 2 µM -
Assay time
3h 00m -
Product overview
cGMP Assay Kit ab65356 provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples.
The cGMP assay protocol uses the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450nm. The intensity of OD450nm is inversely proportional to the concentration of cGMP in samples.
The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04-10 pmol/well (0.008-2 µM) cGMP samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K372 cGMP Direct Immunoassay Kit (Colorimetric). K372-100 is the same size as the 100 test size of ab65356.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Acetylating Reagent A 1 x 750µl Acetylating Reagent B 1 x 1.5ml Anti-cGMP Ab 1 vial Assay Buffer XXXVI 1 x 25ml cGMP Standard 1 vial cGMP-HRP 1 vial Neutralization Buffer III 1 x 7.5ml Protein G Coated Plate 1 pack TMB Substrate I 1 x 10ml -
Research areas
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Relevance
Cyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from guanosine triphosphate (GTP). It serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG). -
Alternative names
- Cyclic GMP
- Cyclic guanosine monophosphate
- Guanosine 3’ 5’ cyclic monophosphate
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab65356 has been referenced in 27 publications.
- Gao W et al. Endothelial nitric oxide synthase (eNOS)-NO signaling axis functions to promote the growth of prostate cancer stem-like cells. Stem Cell Res Ther 13:188 (2022). PubMed: 35526071
- Zabbarova IV et al. Benign prostatic hyperplasia/obstruction ameliorated using a soluble guanylate cyclase activator. J Pathol 256:442-454 (2022). PubMed: 34936088
- Shin D et al. Extracorporeal shock wave therapy combined with engineered mesenchymal stem cells expressing stromal cell-derived factor-1 can improve erectile dysfunction in streptozotocin-induced diabetic rats. Transl Androl Urol 10:2362-2372 (2021). PubMed: 34295723
- Jin YJ et al. Protein kinase N2 mediates flow-induced endothelial NOS activation and vascular tone regulation. J Clin Invest 131:N/A (2021). PubMed: 34499618
- Yang Q et al. Combined Transplantation of Adipose Tissue-Derived Stem Cells and Endothelial Progenitor Cells Improve Diabetic Erectile Dysfunction in a Rat Model. Stem Cells Int 2020:2154053 (2020). PubMed: 32714394