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Abcam's cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450nm. The intensity of OD450nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04-10 pmol/well (0.008-2 µM) cGMP samples.
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|10X cGMP Assay Buffer||WM||1 x 25ml|
|Acetylating Reagent A||Violet||1 x 750µl|
|Acetylating Reagent B||Black||1 x 1.5ml|
|Anti-cGMP pAb/BSA||Red||1 vial|
|HRP Developer||Amber||1 x 10ml|
|Neutralizing Buffer||NM||1 x 7.5ml|
|Protein G Coated Plate||1 pack|
|Standard cGMP (10 nmol)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab65356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
O.D.'s of human urine measured at various dilutions (+/- SD)
Standard curve showing mean of duplicates (+/-SD) with background readings subtracted