Overview

  • Product name
    CGP 57380
  • Description
    MNK inhibitor
  • Biological description
    Low-molecular-weight kinase inhibitor of MAP-kinase interacting kinase-1 (MNK1) and MNK2 (IC50 values are 0.87 and 1.6 μM, respectively). Inhibits a range of other kinases (IC50 values in μM shown in brackets) including: CKI (0.51) Aurora B (2.5) DYRK3 (3.2) SGK1( 2.7) BRSK2 (1.1) and Lck (2.5).
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab120365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab76256 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 (ab120365), by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120365 (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76256 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Bain J  et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
  • Knauf U  et al. Negative regulation of protein translation by mitogen-activated protein kinase-interacting kinases 1 and 2. Mol Cell Biol 21:5500-11 (2001). Read more (PubMed: 11463832) »
  • Tschopp C  et al. Phosphorylation of eIF-4E on Ser 209 in response to mitogenic and inflammatory stimuli is faithfully detected by specific antibodies. Mol Cell Biol Res Commun 3:205-211 (2000). Read more (PubMed: 10891393) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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