Overview

  • Product name
    Anti-CGRP antibody [4901]
    See all CGRP primary antibodies
  • Description
    Mouse monoclonal [4901] to CGRP
  • Specificity
    ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP).
  • Tested applications
    Suitable for: Neutralising, ICC/IF, Flow Cyt, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Mammal
  • Immunogen

    Rat alpha-CGRP

  • Positive control
    • Dorsal root ganglia, pancreas and gut.
  • General notes


    This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
  • Clonality
    Monoclonal
  • Clone number
    4901
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 24430801

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/1000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • Cellular localization
      Endoplasmic reticulum and Secreted
    • Database links
    • Alternative names
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Anti-CGRP antibody [4901] images

    • ab81887 staining rat DRG sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde, permeabilised in 0.3% PBS-Tween and blocked for non-specific protein-protein interactions with 10% donkey's serum for 1h at 24°C. Staining with ab81887 at a 1/1000 dilution in PBS-Triton (0.3%) with 10% donkey's serum and 0.02% azide was performed for 24h at 4°C. A donkey anti-mouse Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody.

      See Abreview

    • Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
    • ab81887 staining CGRP in Mouse dorsal root ganglion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 2% BSA and 2% goat serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in blocking solution) for 12 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (2 mg/ml) was used as the secondary antibody.

      See Abreview

    References for Anti-CGRP antibody [4901] (ab81887)

    This product has been referenced in:
    • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »
    • Minatani A  et al. Activation of calcitonin gene-related peptide signaling through the prostaglandin E2-EP1/EP2/EP4 receptor pathway in synovium of knee osteoarthritis patients. J Orthop Surg Res 11:117 (2016). IHC (PFA fixed) ; Human . Read more (PubMed: 27751171) »

    See all 17 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Nervus Suralis peripheral neurons)
    Permeabilization
    Yes - Triton X-100
    Specification
    Nervus Suralis peripheral neurons
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Fixative
    Acetone
    Username

    Mr. Thomas Klein

    Verified customer

    Submitted Sep 11 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Dorsal root ganglion neuron)
    Permeabilization
    Yes - Triton X
    Specification
    Dorsal root ganglion neuron
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 05 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample
    Rat Tissue sections (Dorsal root ganglian)
    Specification
    Dorsal root ganglian
    Permeabilization
    Yes - 0.3% TritonX-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 16 2015

    Thank you for contacting us,

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    Thank you very much for contacting us with your questions.

    Our antibodies are intended for in vitro use, and so we do not have a protocol for in vivo use or information about the half-life of the antibody. The concentration of ab81887 is lot-...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (DRG neurons)
    Specification
    DRG neurons
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Mar 21 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (dorsal root ganglion)
    Specification
    dorsal root ganglion
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    2% BSA and 2% goat serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    有作 岩崎

    Verified customer

    Submitted Feb 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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