Overview

  • Product name
    Anti-CGRP antibody [4901]
    See all CGRP primary antibodies
  • Description
    Mouse monoclonal [4901] to CGRP
  • Host species
    Mouse
  • Specificity
    ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP).
  • Tested applications
    Suitable for: Neutralising, ICC/IF, Flow Cyt, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Mammal
  • Immunogen

    Rat alpha-CGRP

  • Positive control
    • Dorsal root ganglia, pancreas and gut.
  • General notes


    This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
  • Clonality
    Monoclonal
  • Clone number
    4901
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 24430801

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/1000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • Cellular localization
      Endoplasmic reticulum and Secreted
    • Database links
    • Alternative names
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Images

    • ab81887 staining rat DRG sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde, permeabilised in 0.3% PBS-Tween and blocked for non-specific protein-protein interactions with 10% donkey's serum for 1h at 24°C. Staining with ab81887 at a 1/1000 dilution in PBS-Triton (0.3%) with 10% donkey's serum and 0.02% azide was performed for 24h at 4°C. A donkey anti-mouse Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody.

      See Abreview

    • Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
    • ab81887 staining CGRP in Mouse dorsal root ganglion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 2% BSA and 2% goat serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in blocking solution) for 12 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (2 mg/ml) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Mohd Isa IL  et al. Implantation of hyaluronic acid hydrogel prevents the pain phenotype in a rat model of intervertebral disc injury. Sci Adv 4:eaaq0597 (2018). Read more (PubMed: 29632893) »
    • Zhang CK  et al. VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats. Mol Brain 11:22 (2018). Read more (PubMed: 29650024) »

    See all 29 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us,

    For ab70218:
    DISCOUNT CODE: xxxxxxxxx
    Expiration date: xxxxxxxx


    For ab22623:
    DISCOUNT CODE: xxxxxx
    Expiration date: xxxxxxxxx


    For ab81887:
    DISCOUNT CODE: xx...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up