Anti-CGRP antibody (ab189786)
Key features and details
- Rabbit polyclonal to CGRP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CGRP antibody
See all CGRP primary antibodies -
Description
Rabbit polyclonal to CGRP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CGRP aa 1 to the C-terminus.
Database link: P06881 -
Positive control
- WB: Mouse brain ICC/IF: Human U2OS cells, Rat C6 cells, Mouse L929 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 14 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 14 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. -
Cellular localization
Endoplasmic reticulum and Secreted -
Database links
- Entrez Gene: 796 Human
- Entrez Gene: 797 Human
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- Omim: 114160 Human
see all -
Alternative names
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
Images
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Anti-CGRP antibody (ab189786) at 1/1000 dilution + Mouse brain at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 14 kDa
Exposure time: 180 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of Human U2OS cells using Anti-CGRP antibody (ab189786) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of Rat C6 cells using Anti-CGRP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of Mouse L929 cells using Anti-CGRP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab189786 has been referenced in 5 publications.
- Shen L et al. Electroacupuncture Modulates 5-HT4R-Mediated cAMP/PKA Signaling to Improve Intestinal Motility Disorders in a Thy1-αSyn Parkinson's Mouse Model. Evid Based Complement Alternat Med 2022:8659462 (2022). PubMed: 36337584
- Wen Q et al. MicroRNA-155-5p promotes neuroinflammation and central sensitization via inhibiting SIRT1 in a nitroglycerin-induced chronic migraine mouse model. J Neuroinflammation 18:287 (2021). PubMed: 34893074
- La Montanara P et al. Cyclin-dependent-like kinase 5 is required for pain signaling in human sensory neurons and mouse models. Sci Transl Med 12:N/A (2020). PubMed: 32641489
- Jasim H et al. Saliva as a medium to detect and measure biomarkers related to pain. Sci Rep 8:3220 (2018). PubMed: 29459715
- Das V et al. Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain. Gene 655:1-12 (2018). PubMed: 29474860