Overview

  • Product name
    Anti-CGRP antibody
    See all CGRP primary antibodies
  • Description
    Goat polyclonal to CGRP
  • Host species
    Goat
  • Specificity
    Antibody is thought to react with whole molecule (1-37) and 23-37 fragment (C term). Specifically visualises CGRP in brain cells.
  • Tested applications
    Suitable for: IHC-FoFr, ELISA, IHC-P, IHC-Frmore details
    Unsuitable for: IHC-R
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat CGRP (C terminal).
    Sequence:

    VKDNFVPTNVGSEAF

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab36001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 19805319
ELISA 1/500 - 1/2500.
IHC-P 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/200.
  • Application notes
    Is unsuitable for IHC-R.

    • Target

    • Relevance
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • Cellular localization
      Endoplasmic reticulum and Secreted
    • Database links
      see all
    • Alternative names
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab36001)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

      IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-CGRP antibody (ab36001)
      Immunohistochemistry (Frozen sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an anonymous Abreview

      ab36001 staining mouse colon tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C.  The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.

      See Abreview

    References

    This product has been referenced in:
    • Liang CC  et al. Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats. Sci Rep 8:2105 (2018). IHC ; Rat . Read more (PubMed: 29391467) »
    • Wei D  et al. Orai1 and Orai3 Mediate Store-Operated Calcium Entry Contributing to Neuronal Excitability in Dorsal Root Ganglion Neurons. Front Cell Neurosci 11:400 (2017). IHC-FoFr ; Mouse . Read more (PubMed: 29311831) »

    See all 30 Publications for this product

    Publishing research using ab36001? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CGRP antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Dorsal Root Ganglia)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Dorsal Root Ganglia
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Jean-Marc Guedon

    Verified customer

    Submitted Jun 03 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 25 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 06 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain, spinal cord, muscle w/nerve)
    Specification
    brain, spinal cord, muscle w/nerve
    Fixative
    formalin, 10% neutral buffered
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6, microwave 2x, 5 min on high
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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