Synthetic peptide corresponding to Rat CGRP (C terminal).
Sequence:
VKDNFVPTNVGSEAF
Our Abpromise guarantee covers the use of ab36001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-FoFr | Use at an assay dependent dilution. PubMed: 19805319 | |
ELISA | 1/500 - 1/2500. | |
IHC-P | 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
IHC-Fr | 1/200. |
IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
ab36001 staining mouse colon tissue sections by IHC-Fr. Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C. The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.
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