The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 19805319
1/500 - 1/2500.
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for IHC-R.
RelevanceCGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
Cellular localizationEndoplasmic reticulum and Secreted
Calcitonin/calcitonin related polypeptide alpha antibody
Alpha type CGRP antibody
Beta type CGRP antibody
Calcitonin 1 antibody
Calcitonin 2 antibody
Calcitonin gene related peptide I antibody
Calcitonin gene related peptide II antibody
Calcitonin related polypeptide alpha antibody
Calcitonin related polypeptide beta antibody
CGRP I antibody
CGRP II antibody
Anti-CGRP antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
Immunohistochemistry (Frozen sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an anonymous Abreview
ab36001 staining mouse colon tissue sections by IHC-Fr. Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C. The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.
Deiteren A et al. P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization. PLoS One10:e0123810 (2015).
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Mathews MA et al. Efferent Vestibular Neurons Show Homogenous Discharge Output But Heterogeneous Synaptic Input Profile In Vitro. PLoS One10:e0139548 (2015).
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