- Datasheet
- Specific References (18)
- Protocols
Overview
- Product nameAnti-CGRP antibody
See all CGRP primary antibodies - DescriptionGoat polyclonal to CGRP
- SpecificityAntibody is thought to react with whole molecule (1-37) and 23-37 fragment (C term). Specifically visualises CGRP in brain cells.
- Tested applicationsSuitable for:
IHC-FoFr, ELISA, IHC-P, IHC-Frmore details
Unsuitable for: IHC-R - Species reactivityReacts with: Mouse, Rat
- Immunogen
Synthetic peptide corresponding to Rat CGRP (C terminal).
Sequence:VKDNFVPTNVGSEAF
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading... - PurityProtein G purified
- Purification notesPurified Ig by Ammonium Sulphate fractionation.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab36001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent dilution. PubMed: 19805319 | |
| ELISA | 1/500 - 1/2500. | |
| IHC-P | 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/200. |
Target
- RelevanceCGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
- Cellular localizationEndoplasmic reticulum and Secreted
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Database links
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- SwissProt: P70160 Mouse
- SwissProt: Q99JA0 Mouse
- SwissProt: P01256 Rat
- SwissProt: P01257 Rat
see all -
Alternative names
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
Anti-CGRP antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
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Immunohistochemistry (Frozen sections) - Anti-CGRP antibody (ab36001)This image is courtesy of an anonymous Abreviewab36001 staining mouse colon tissue sections by IHC-Fr. Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C. The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.
References for Anti-CGRP antibody (ab36001)
This product has been referenced in:
- Deiteren A et al. P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization. PLoS One 10:e0123810 (2015). IHC-FoFr ; Rat . Read more (PubMed: 25885345) »
- Mathews MA et al. Efferent Vestibular Neurons Show Homogenous Discharge Output But Heterogeneous Synaptic Input Profile In Vitro. PLoS One 10:e0139548 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26422206) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"