Overview

  • Product nameAnti-CGRP antibody
    See all CGRP primary antibodies
  • Description
    Goat polyclonal to CGRP
  • SpecificityAntibody is thought to react with whole molecule (1-37) and 23-37 fragment (C term). Specifically visualises CGRP in brain cells.
  • Tested applicationsSuitable for: IHC-FoFr, ELISA, IHC-P, IHC-Frmore details
    Unsuitable for: IHC-R
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat CGRP (C terminal).
    Sequence:

    VKDNFVPTNVGSEAF

Properties

Applications

Our Abpromise guarantee covers the use of ab36001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 19805319
ELISA 1/500 - 1/2500.
IHC-P 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/200.
  • Application notesIs unsuitable for IHC-R.
  • Target

    • RelevanceCGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • Cellular localizationEndoplasmic reticulum and Secreted
    • Database links
    • Alternative names
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Anti-CGRP antibody images

    • IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.

      See Abreview

    • ab36001 staining mouse colon tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C.  The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.

      See Abreview

    References for Anti-CGRP antibody (ab36001)

    This product has been referenced in:
    • Deiteren A  et al. P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization. PLoS One 10:e0123810 (2015). IHC-FoFr ; Rat . Read more (PubMed: 25885345) »
    • Bäumer BE  et al. Nogo receptor homolog NgR2 expressed in sensory DRG neurons controls epidermal innervation by interaction with Versican. J Neurosci 34:1633-46 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24478347) »

    See all 15 Publications for this product

    Product Wall

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Dorsal Root Ganglia)
    Antigen retrieval step None
    Permeabilization Yes - 0.3% Triton X-100
    Specification Dorsal Root Ganglia
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Dr. Jean-Marc Guedon

    Verified customer

    Submitted Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Spinal cord)
    Specification Spinal cord
    Fixative Formaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 25 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Spinal cord)
    Specification Spinal cord
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 06 2012

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (brain, spinal cord, muscle w/nerve)
    Specification brain, spinal cord, muscle w/nerve
    Fixative formalin, 10% neutral buffered
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, pH6, microwave 2x, 5 min on high
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 18 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Colon)
    Specification Colon
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"