Overview

  • Product name
  • Description
    Rabbit polyclonal to CGRP
  • Specificity
    The immunogen used to raise this antibody has 78% homology with the human CGRP protein. Previous batches of ab47027 have shown to work in human tissue. Please contact Abcam Scientific Support for more information.
  • Tested applications
    Suitable for: IHC-P, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat CGRP.

    (Peptide available as ab47101.)

  • Positive control
    • IHC-P: FFPE rat dorsal root ganglion and rat thyroid tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab47027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-P Use a concentration of 0.1 - 5 µg/ml.
WB 1/500. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa). PubMed: 27282805

Target

  • Relevance
    CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
  • Cellular localization
    Endoplasmic reticulum and Secreted
  • Database links
  • Alternative names
    • Calcitonin/calcitonin related polypeptide alpha antibody
    • Alpha type CGRP antibody
    • Beta type CGRP antibody
    • CALC1 antibody
    • CALC2 antibody
    • CALCA antibody
    • CALCB antibody
    • Calcitonin 1 antibody
    • Calcitonin 2 antibody
    • Calcitonin gene related peptide I antibody
    • Calcitonin gene related peptide II antibody
    • Calcitonin related polypeptide alpha antibody
    • Calcitonin related polypeptide beta antibody
    • CGRP antibody
    • CGRP I antibody
    • CGRP II antibody
    • CGRP1 antibody
    • CGRP2 antibody
    • CT antibody
    • Katacalcin antibody
    • KC antibody
    see all

Anti-CGRP antibody images

  • IHC image of CGRP staining in a Rat normal thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of CGRP staining in rat dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of CGRP staining in rat dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC-P image of CGRP staining on Rat spinal cord sections using ab47027 (1:400). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.

    See Abreview

References for Anti-CGRP antibody (ab47027)

This product has been referenced in:
  • Xu Y  et al. Mechanisms of PDGF siRNA-mediated inhibition of bone cancer pain in the spinal cord. Sci Rep 6:27512 (2016). WB ; Rat . Read more (PubMed: 27282805) »
  • Li ZW  et al. Brain natriuretic peptide suppresses pain induced by BmK I, a sodium channel-specific modulator, in rats. J Headache Pain 17:90 (2016). WB ; Rat . Read more (PubMed: 27687165) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain tissue)
Specification
Brain tissue
Username

Abcam user community

Verified customer

Submitted Apr 07 2017

Thank you for contacting us. I would be very happy to refund these products for you. I have only found orders for 1 vial of this product associated with your user account. This is for order number xxxxx. Could you please provide the Abcam order c...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (tongue)
Specification
tongue
Username

Abcam user community

Verified customer

Submitted Sep 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2012



I am very pleased to hear your customer would like to test ab47027 Anti-CGRP antibody in rabbit. This code will give them1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for rabbit and include...

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Thank you for contacting us.


I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a di...

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Thank you for your enquiry.

I can confirm that the immunogen for ab47027spans part of the signal peptide and the first propeptide so it should only detect full length unprocessed CGRP.

The immunogen sequence is:
#############...

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Thank you for taking the timetotry the suggestions.

I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody again. I appreciate the time spent in the laboratory and understand the concerns. It is ...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. The details your customer has kindly provided will e...

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Thank you for your patience while I looked into the lysate used for testing this antibody.  Here is the lab's reply: "The lysate we use for testing this antibody is actually supplied by a collaborator.  I don’t have a lot of information about the l...

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