The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
Immunohistochemical analysis of expression of CGRP using ab92609.
A: the expression of CGRP is shown in the entire trigeminal ganglion obtained from an untreated adult rat.
B: at higher magnification (bar = 20µm), neuronal processes (arrows) expressing CGRP that extend from neuronal cell bodies in one cluster to another cluster are observed.
Protocol: ab92609 was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature.Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with 1/100 ab92609. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.