Overview

  • Product name
  • Description
    Chicken polyclonal to CGRP
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide:

    KDNFVPTNVGSEAF-NH2

    , corresponding to amino acids 106-119 of Rat CGRP (SwissProt ID: P01256)

  • Positive control
    • Trigeminal ganglion neurons

Properties

Applications

Our Abpromise guarantee covers the use of ab92609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • Cellular localization
      Endoplasmic reticulum and Secreted
    • Database links
    • Alternative names
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Images

    • Immunohistochemical analysis of expression of CGRP using ab92609.
      A: the expression of CGRP is shown in the entire trigeminal ganglion obtained from an untreated adult rat.
      B: at higher magnification (bar = 20µm), neuronal processes (arrows) expressing CGRP that extend from neuronal cell bodies in one cluster to another cluster are observed.

      Protocol: ab92609 was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature.Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with 1/100 ab92609. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

    References

    ab92609 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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