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All lanes : Anti-ch TOG antibody (ab86073) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : mouse NIH3T3 cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 218 kDa
Exposure time : 30 seconds
Immunoprecipitation/ Western Blot of ab86073. Lane 1: ab86073 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab86073 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
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