Anti-ch TOG antibody (ab86073)
Key features and details
- Rabbit polyclonal to ch TOG
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ch TOG antibody
See all ch TOG primary antibodies -
Description
Rabbit polyclonal to ch TOG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human ch TOG.
Database link: Q14008 -
Positive control
- HeLa whole cell lysate (ab150035), NIH3T3 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 218 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin. -
Cellular localization
Localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis -
Database links
- Entrez Gene: 9793 Human
- Entrez Gene: 75786 Mouse
- Entrez Gene: 311191 Rat
- Omim: 611142 Human
- SwissProt: Q0VAX7 Human
- SwissProt: Q0VAX8 Human
- SwissProt: Q14008 Human
- SwissProt: Q8IYN5 Human
see all -
Alternative names
- Ch TOG protein antibody
- chTOG antibody
- CHTOG protein antibody
see all
Images
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All lanes : Anti-ch TOG antibody (ab86073) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 218 kDa
Exposure time: 30 seconds -
Immunoprecipitation/ Western Blot of ab86073. Lane 1: ab86073 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab86073 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab86073 has been referenced in 7 publications.
- Serpico AF et al. Compartmentalized control of Cdk1 drives mitotic spindle assembly. Cell Rep 38:110305 (2022). PubMed: 35081344
- Liu X et al. CircPDSS1 promotes the proliferation, invasion, migration, and EMT of breast cancer cell via regulating miR-320c/CKAP5 axis. Cancer Cell Int 22:238 (2022). PubMed: 35902921
- Huang D et al. GLI2 promotes cell proliferation and migration through transcriptional activation of ARHGEF16 in human glioma cells. J Exp Clin Cancer Res 37:247 (2018). PubMed: 30305138
- Zhang Y et al. The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression. J Biol Chem 293:15733-15747 (2018). PubMed: 30054275
- Gulluni F et al. Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2a Scaffolding Function. Cancer Cell 32:444-459.e7 (2017). PubMed: 29017056
- Wordeman L et al. Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins. Mol Biol Cell 27:1300-9 (2016). WB . PubMed: 26912793
- Borner GH et al. Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles. J Cell Biol 197:141-60 (2012). WB . PubMed: 22472443