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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-ch TOG antibody
See all ch TOG products (2) ...
Description
Rabbit polyclonal to ch TOG
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A region between residue 1982 and 2032 of Human ch TOG (NP_001008938.1).
Positive control
HeLa whole cell lysate, NIH3T3 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Predicted molecular weight: 218 kDa.
IP: Use at 2-5 µg/mg of lysate.
Target
Relevance
ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin.
Cellular localization
Localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis
Alternative names
- Ch TOG protein antibody
- chTOG antibody
- CHTOG protein antibody
see all
Database links
- Entrez Gene: 9793 Human
- Entrez Gene: 75786 Mouse
- Omim: 611142 Human
- SwissProt: Q0VAX7 Human
- SwissProt: Q0VAX8 Human
- SwissProt: Q14008 Human
- SwissProt: Q8IYN5 Human
- SwissProt: Q0VGR0 Mouse
see all
Anti-ch TOG antibody images:
Western blot - ch TOG antibody (ab86073)
All lanes : Anti-ch TOG antibody (ab86073) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : mouse NIH3T3 cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 218 kDa
Exposure time : 30 seconds
Immunoprecipitation - ch TOG antibody (ab86073)
Immunoprecipitation/ Western Blot of ab86073. Lane 1: ab86073 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab86073 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
References for Anti-ch TOG antibody (ab86073)
ab86073 has not yet been referenced specifically in any publications.
Publishing research using ab86073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"