Overview

  • Product name
    Anti-ch TOG antibody
  • Description
    Rabbit polyclonal to ch TOG
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    A region between residue 1982 and 2032 of Human ch TOG (NP_001008938.1).

  • Positive control
    • HeLa whole cell lysate, NIH3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin.
  • Cellular localization
    Localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis
  • Database links
  • Alternative names
    • Ch TOG protein antibody
    • chTOG antibody
    • CHTOG protein antibody
    • CKAP 5 antibody
    • CKAP5 antibody
    • CKAP5 protein antibody
    • Colonic and hepatic tumor over expressed antibody
    • Colonic and hepatic tumor over expressed protein antibody
    • Colonic and hepatic tumor overexpressed protein antibody
    • Cytoskeleton associated protein 5 antibody
    • FLJ35359 antibody
    • KIAA0097 antibody
    • TOG antibody
    • TOGp antibody
    see all

Images

  • All lanes : Anti-ch TOG antibody (ab86073) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : mouse NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 218 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation/ Western Blot of ab86073. Lane 1: ab86073 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab86073 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:
  • Wordeman L  et al. Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins. Mol Biol Cell 27:1300-9 (2016). WB . Read more (PubMed: 26912793) »
  • Borner GH  et al. Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles. J Cell Biol 197:141-60 (2012). WB . Read more (PubMed: 22472443) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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