Overview

  • Product nameAnti-CH25H antibody
    See all CH25H primary antibodies
  • Description
    Mouse monoclonal to CH25H
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 142-247 of Human CH25H (AAH17843) with a 26 kDa enzyme tag.

  • Positive control
    • Recombinant CH25H fragment

Properties

Applications

Our Abpromise guarantee covers the use of ab76478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 13 kDa plus 26 kDa tag).
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFunction: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Similarity: Belongs to the sterol desaturase family. PTM: N-glycosylated.
    • Cellular localizationEndoplasmic reticulum membrane
    • Database links
    • Alternative names
      • C25H antibody
      • Ch25h antibody
      • CH25H_HUMAN antibody
      • Cholesterol 25 hydroxylase antibody
      • Cholesterol 25 monooxygenase antibody
      • Cholesterol 25-hydroxylase antibody
      • Cholesterol 25-monooxygenase antibody
      • EC 1.14.99.38 antibody
      • EC=1.14.99.38 antibody
      • h25OH antibody
      see all

    Anti-CH25H antibody images

    • Anti-CH25H antibody (ab76478) at 5 µg/ml + recombinant fragment of CH25H (plus 26 kDa tag) at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 13 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-CH25H antibody (ab76478)

    ab76478 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Liver tissue)
    Loading amount 50 µg
    Specification Liver tissue
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Feb 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"