Overview

  • Product name
  • Description
    Rabbit polyclonal to CHAC1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A synthetic peptide corresponding to a region of Human CHAC1

  • Positive control
    • 721_B cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution (PubMed:23070364). Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).
  • Sequence similarities
    Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.
  • Cellular localization
    Cytoplasm, cytosol. Golgi apparatus, trans-Golgi network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blocks Notch protein antibody
    • Botch antibody
    • Cation transport regulator like protein 1 antibody
    • Cation transport regulator-like protein 1 antibody
    • ChaC antibody
    • ChaC cation transport regulator homolog 1 antibody
    • ChaC cation transport regulator like 1 antibody
    • chac1 antibody
    • CHAC1_HUMAN antibody
    • Gamma GCT acting on glutathione homolog 1 antibody
    • Gamma-GCG 1 antibody
    • Glutathione-specific gamma-glutamylcyclotransferase 1 antibody
    see all

Images

  • Anti-CHAC1 antibody (ab76386) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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