Overview

  • Product name
  • Description
    Rabbit polyclonal to CHADL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 493-620 of Human CHADL.

  • Positive control
    • Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class IV subfamily.
    Contains 19 LRR (leucine-rich) repeats.
    Contains 2 LRRCT domains.
    Contains 2 LRRNT domains.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AY100452 antibody
    • CHADL antibody
    • CHADL_HUMAN antibody
    • Chondroadherin-like protein antibody
    • D930017K21Rik antibody
    • mCG_8786 antibody
    • RP4-756G23.1 antibody
    • SLRR4B antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121228 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121228 at 1/10- dilution staining CHADL in Paraffin-embedded Human tonsil tissue by Immunohistochemistry.

References

ab121228 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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