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Abcam’s anti-Chagas IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Chagas in Human serum and plasma.
A 96-well plate has been precoated with Chagas antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Chagas antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Chagas IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Chagas Anti-IgG HRP Conjugate||1 x 20ml|
|Chagas Coated Microplate (12 x 8 wells)||1 unit|
|Chagas Cut-off Control||1 x 3ml|
|Chagas Negative Control||1 x 2ml|
|Chagas Positive Control||1 x 2ml|
|Cover Foil||1 unit|
|IgG Sample Diluent||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab178637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178637 has not yet been referenced specifically in any publications.