Overview

  • Product name
  • Description
    Rabbit polyclonal to CHCHD10
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding amino acids 43-142 (GLMAQMATTA AGVAVGSAVG HVMGSALTGA FSGGSSEPSQ PAVQQAPTPA APQPLQMGPC AYEIRQFLDC STTQSDLSLC EGFSEALKQC KYYHGLSSLP) of Human CHCHD10.

  • Positive control
    • Human kidney tissue; RT-4 and Human tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Contains 1 CHCH domain.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • C22orf16 antibody
    • CHC10_HUMAN antibody
    • CHCHD10 antibody
    • Chromosome 22 open reading frame 16 antibody
    • Coiled coil helix coiled coil helix domain containing 10 antibody
    • Coiled coil helix coiled coil helix domain containing protein 10 mitochondrial antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 10 antibody
    • MGC70831 antibody
    • mitochondrial antibody
    • N27C7-4 antibody
    • OTTHUMP00000198408 antibody
    • OTTHUMP00000198409 antibody
    • Protein N27C7-4 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121196 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-CHCHD10 antibody (ab121196) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121196, at 1/200 dilution, staining CHCHD10 in paraffin-embedded Human kidney tissue, by Immunohistochemistry.

References

ab121196 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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