Overview

  • Product nameAnti-CHCHD3 antibody
    See all CHCHD3 primary antibodies
  • Description
    Mouse polyclonal to CHCHD3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length human CHCHD3 protein (NP_060282).

  • Positive control
    • Human placenta tissue lysate and CHCHD3 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • FunctionRequired for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.
  • Tissue specificityDetected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
  • Sequence similaritiesContains 1 CHCH domain.
  • Cellular localizationMitochondrion inner membrane. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHCH3_HUMAN antibody
    • CHCHD 3 antibody
    • Chchd3 antibody
    • Coiled coil helix coiled coil helix domain containing 3 antibody
    • Coiled coil helix coiled coil helix domain containing protein 3 mitochondrial antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 antibody
    • FLJ20420 antibody
    • MINOS3 antibody
    • mitochondrial antibody
    • Mitochondrial inner membrane organizing system 3 antibody
    • PPP1R22 antibody
    • Protein phosphatase 1 regulatory subunit 22 antibody
    see all

Anti-CHCHD3 antibody images

  • Anti-CHCHD3 antibody (ab69328) at 1/500 dilution + Human placenta tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • All lanes : Anti-CHCHD3 antibody (ab69328) at 1/500 dilution

    Lane 1 : CHCHD3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 26 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-CHCHD3 antibody (ab69328)

This product has been referenced in:
  • Ioakeimidis F  et al. A splicing mutation in the novel mitochondrial protein DNAJC11 causes motor neuron pathology associated with cristae disorganization, and lymphoid abnormalities in mice. PLoS One 9:e104237 (2014). WB ; Mouse . Read more (PubMed: 25111180) »
  • An J  et al. CHCM1/CHCHD6, Novel Mitochondrial Protein Linked to Regulation of Mitofilin and Mitochondrial Cristae Morphology. J Biol Chem 287:7411-26 (2012). Read more (PubMed: 22228767) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"