Overview

  • Product nameAnti-Chd1 antibody
  • Description
    Rabbit polyclonal to Chd1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Chd1 aa 1332-1381 (internal sequence).
    Sequence:

    RKARAKKNKAMKSIKVKEEIKSDSSPLPSEKSDEDDDKLSESKSDGRERS


    Database link: O14646

  • Positive control
    • Human fetal stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 197 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3).
  • Cellular localizationNucleus. Cytoplasm. Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase CHD 1 antibody
    • ATP-dependent helicase CHD1 antibody
    • CHD 1 antibody
    • CHD-1 antibody
    • Chd1 antibody
    • CHD1_HUMAN antibody
    • Chromodomain helicase DNA binding protein 1 antibody
    • Chromodomain-helicase-DNA-binding protein 1 antibody
    • DKFZp686E2337 antibody
    • OTTHUMP00000222625 antibody
    see all

Anti-Chd1 antibody images

  • Anti-Chd1 antibody (ab94848) at 1 µg/ml + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 197 kDa

References for Anti-Chd1 antibody (ab94848)

ab94848 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"