Overview

  • Product nameAnti-CHD1L antibody [2170C3a]
    See all CHD1L primary antibodies
  • Description
    Mouse monoclonal [2170C3a] to CHD1L
  • Tested applicationsSuitable for: WB, IP, Dot blot, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human CHD1L fragment

  • Positive control
    • HEK293 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab51324 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ClonalityMonoclonal
  • Clone number2170C3a
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab51324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Dot blot
IHC-P
  • Application notesDot: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution (PMID 20335658).
    IP: Use at 10µg/mg RIPA lysate of HeLa cells.
    WB: 1/50. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
    • Tissue specificityFrequently overexpressed in hepatomacellular carcinomas.
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 Macro domain.
    • DomainThe macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
    • Cellular localizationNucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALC1 antibody
      • Amplified in liver cancer 1 antibody
      • Amplified in liver cancer protein 1 antibody
      • chd1l antibody
      • CHD1L_HUMAN antibody
      • CHDL antibody
      • Chromodomain helicase DNA binding protein 1 like antibody
      • Chromodomain-helicase-DNA-binding protein 1-like antibody
      • FLJ22530 antibody
      see all

    Anti-CHD1L antibody [2170C3a] images

    • Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 101 kDa
      Observed band size : 101 kDa
    • Immunoprecipitation analysis using ab51324 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uisng ab51324 at 1/50 dilution and 1/2500 anti-mouse IgG as secondary.
    • IHC image of ab51324 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51324, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-CHD1L antibody [2170C3a] (ab51324)

    This product has been referenced in:
    • Chen L  et al. Clinical significance of CHD1L in hepatocellular carcinoma and therapeutic potentials of virus-mediated CHD1L depletion. Gut 60:534-43 (2011). WB ; Human . Read more (PubMed: 21068133) »
    • Chen L  et al. CHD1L promotes hepatocellular carcinoma progression and metastasis in mice and is associated with these processes in human patients. J Clin Invest 120:1178-91 (2010). IHC-P ; Mouse . Read more (PubMed: 20335658) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (cancer cell lines)
    Specification cancer cell lines
    Blocking step cold fish geletin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (7.5)
    Sample Human Cell lysate - whole cell (Hela cells)
    Specification Hela cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Mr. Peter Rüthemann

    Verified customer

    Submitted Jul 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"