The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionDNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Tissue specificityFrequently overexpressed in hepatomacellular carcinomas.
Detection of CHD1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118799 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-CHD1L antibody which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-CHD1L antibody (ab118799)
has not yet been referenced specifically in any publications.
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