Overview

  • Product name
  • Description
    Rabbit polyclonal to CHD1L
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 847-897 of Human CHD1L (NP_004275.3).

  • Positive control
    • 293T whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab118799 was affinity purified using an epitope specific to CHD1L immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
    • Tissue specificity
      Frequently overexpressed in hepatomacellular carcinomas.
    • Sequence similarities
      Belongs to the SNF2/RAD54 helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 Macro domain.
    • Domain
      The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
    • Cellular localization
      Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALC1 antibody
      • Amplified in liver cancer 1 antibody
      • Amplified in liver cancer protein 1 antibody
      • chd1l antibody
      • CHD1L_HUMAN antibody
      • CHDL antibody
      • Chromodomain helicase DNA binding protein 1 like antibody
      • Chromodomain-helicase-DNA-binding protein 1-like antibody
      • FLJ22530 antibody
      see all

    Images

    • Detection of CHD1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118799 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-CHD1L antibody which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab118799 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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