The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Frequently overexpressed in hepatomacellular carcinomas.
Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 Macro domain.
The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
Detection of CHD1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118799 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-CHD1L antibody which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.