Overview

  • Product nameAnti-CHD1L antibody
    See all CHD1L primary antibodies
  • Description
    Rabbit polyclonal to CHD1L
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: KDYSKEPSKE DRKSFEQLVN LQKTLLEKAS QEGRSLRNKG SVLIPGLVEG, corresponding to internal sequence amino acids 578-627 of Human CHD1L.

  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab33042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 101 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionDNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
  • Tissue specificityFrequently overexpressed in hepatomacellular carcinomas.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 Macro domain.
  • DomainThe macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
  • Cellular localizationNucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALC1 antibody
    • Amplified in liver cancer 1 antibody
    • Amplified in liver cancer protein 1 antibody
    • chd1l antibody
    • CHD1L_HUMAN antibody
    • CHDL antibody
    • Chromodomain helicase DNA binding protein 1 like antibody
    • Chromodomain-helicase-DNA-binding protein 1-like antibody
    • FLJ22530 antibody
    see all

References for Anti-CHD1L antibody (ab33042)

ab33042 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (Human cancer lines)
Specification Human cancer lines
Blocking step cold fish geletin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"