The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for WB.
FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ewing sarcoma (left) and mouse renal cell carcinoma (right) tissues labelling CHD3 with ab114100 at 1/1000 (1µg/ml). Detection: DAB.
Rabbit anti-CHD3 antibody, at 1 µg/ml, staining CHD3 in HeLa whole cell lysate immunoprecipitated using ab114100 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-CHD3 antibody (ab114100)
has not yet been referenced specifically in any publications.
Publishing research using ab114100? Please let us know so that we can cite the reference in this datasheet.
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