Overview

  • Product nameAnti-CHD3 antibody
    See all CHD3 primary antibodies
  • Description
    Rabbit polyclonal to CHD3
  • Tested applicationsSuitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1950-2000 of Human CHD3 (NP_001005273.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab114100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 226 antibody
      • 2600010P09Rik antibody
      • AF020312 antibody
      • ATP dependent helicase CHD3 antibody
      • ATP-dependent helicase Chd3 antibody
      • CHD-3 antibody
      • Chd3 antibody
      • CHD3_HUMAN antibody
      • Chd7 antibody
      • Chromodomain-helicase-DNA-binding protein 3 antibody
      • hZFH antibody
      • MGC40857 antibody
      • Mi 2 autoantigen 240 kDa protein antibody
      • Mi 2a antibody
      • Mi-2 autoantigen 240 kDa protein antibody
      • Mi2 ALPHA antibody
      • Mi2-alpha antibody
      • Prp7 antibody
      • Prp9 1 antibody
      • ZFH antibody
      • Zinc finger helicase antibody
      • zinc-finger helicase (Snf2-like) antibody
      see all

    Anti-CHD3 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ewing sarcoma (left) and mouse renal cell carcinoma (right) tissues labelling CHD3 with ab114100 at 1/1000 (1µg/ml). Detection: DAB.
    • Rabbit anti-CHD3 antibody, at 1 µg/ml, staining CHD3 in HeLa whole cell lysate immunoprecipitated using ab114100 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-CHD3 antibody (ab114100)

    ab114100 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"