Overview

  • Product name
  • Description
    Rabbit polyclonal to CHD3
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1950 and 2000 of Human CHD3 (NP_001005273.1).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Ewing Sarcoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Squamous Cell Carcinoma and Small Cell Lung Cancer tissue. Mouse Teratoma and Renal Cell Carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab84528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.

Target

  • Function
    Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 226 antibody
    • 2600010P09Rik antibody
    • AF020312 antibody
    • ATP dependent helicase CHD3 antibody
    • ATP-dependent helicase Chd3 antibody
    • CHD-3 antibody
    • Chd3 antibody
    • CHD3_HUMAN antibody
    • Chd7 antibody
    • Chromodomain-helicase-DNA-binding protein 3 antibody
    • hZFH antibody
    • MGC40857 antibody
    • Mi 2 autoantigen 240 kDa protein antibody
    • Mi 2a antibody
    • Mi-2 autoantigen 240 kDa protein antibody
    • Mi2 ALPHA antibody
    • Mi2-alpha antibody
    • Prp7 antibody
    • Prp9 1 antibody
    • ZFH antibody
    • Zinc finger helicase antibody
    • zinc-finger helicase (Snf2-like) antibody
    see all

Anti-CHD3 antibody images

  • ab84528, at 1/250 dilution, staining CHD3 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
  • ab84528, at 1/250 dilution, staining CHD3 in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry using DAB staining.
  • ab84528, at 1/250 dilution, staining CHD3 in formalin-fixed, paraffin-embedded mouse renal cell carcinoma by Immunohistochemistry using DAB staining.

References for Anti-CHD3 antibody (ab84528)

This product has been referenced in:
  • Yamashita D  et al. Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2a. J Biol Chem 291:11619-34 (2016). Read more (PubMed: 27068747) »
  • Klement K  et al. Opposing ISWI- and CHD-class chromatin remodeling activities orchestrate heterochromatic DNA repair. J Cell Biol 207:717-33 (2014). Read more (PubMed: 25533843) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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